May 10, 2016 22:10
8 yrs ago
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английский term
molar equivalent to antibody was added to the antibody
английский => русский
Наука
Биология (биотехника, биохимия, микробиология)
The ADCs were prepared as follows. The starting antibody solution was loaded
onto the PD-10 column, previously equilibrated with 25 mL of Conjugation Buffer 1, followed by 0.5 ml Conjugation Buffer 1. The antibody eluate was collect and the concentration measured at A28o and the concentration was adjusted to 20 mg/mL. The 10 mM SMCC-DM1 solution in DMA was prepared. ""A 7.5 molar equivalent of SMCC-DM1 to antibody was added to the antibody"" solution and DMA was added to a final DMA volume of 10% v/v. The reaction was briefly mixed and incubated at RT for 2 h.
Спасибо!
onto the PD-10 column, previously equilibrated with 25 mL of Conjugation Buffer 1, followed by 0.5 ml Conjugation Buffer 1. The antibody eluate was collect and the concentration measured at A28o and the concentration was adjusted to 20 mg/mL. The 10 mM SMCC-DM1 solution in DMA was prepared. ""A 7.5 molar equivalent of SMCC-DM1 to antibody was added to the antibody"" solution and DMA was added to a final DMA volume of 10% v/v. The reaction was briefly mixed and incubated at RT for 2 h.
Спасибо!
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May 10, 2016 22:10: changed "Kudoz queue" from "In queue" to "Public"
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A 7.5 molar equivalent of SMCC-DM1 to antibody was added to the antibody\"\" solution = К раствору антитела добавляли SMCC-DM1 в молярном соотношении к антителу 7.5
A 7.5 molar equivalent of SMCC-DM1 to antibody was added to the antibody\"\" solution = К раствору антитела добавляли SMCC-DM1 в молярном соотношении к антителу 7.5
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